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pci sep nr2b  (Addgene inc)


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    Structured Review

    Addgene inc pci sep nr2b
    Pci Sep Nr2b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pci+sep+nr2b/pCI-SEP_NR2B+(Plasmid+%2323998)/pm40716012-42-8-12
    Average 93 stars, based on 15 article reviews
    pci sep nr2b - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Clustering of Tau fibrils impairs the synaptic composition of α3‐Na + /K + ‐ ATP ase and AMPA receptors
    Article Snippet: .. Plasmid Source **Amount /Transfection pCI-SEP-NRI (Addgene item #23999) (Kopec et al, 2006) 0.75μg pCI-SEP-NR2B (Addgene item #23998) (Kopec et al, 2006) 0.75μg pCI-SEP-GluR1 (Addgene item #24000) (Kopec et al, 2006) 0.5μg pCI-SEP GluR2(R) (Addgene item #24001) (Kopec et al, 2006) 0.5μg α3-NKA-Dendra2 SEP replaced with Dendra2 (Azarias et al, 2013) 0.5μg mRFP-Clc (Clathrin) (Addgene item #14435) (Tagawa et al, 2005) 0.4μg SEP = Super-ecliptic pHluorin Cell-surface biotinylation Cell surface biotinylation experiment was performed using Pierce Cell Surface Protein Isolation Kit (Thermo Fischer Scientific) as per manufacturer’s instructions. ..

    Article Title: TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation
    Article Snippet: Transient transfection of the HEK293 cells was performed using Lipofectamine 2000 (Invitrogen) after 24 hours (34). .. Before the transfection experiment, the cells were rinsed twice with warm PBS (pH 7.4) and subsequently transfected with 2 μg of pCI-SEP-NR2B (Addgene, plasmid 23998) and mCherryTM25 (GeneChem) on day in vitro (DIV) 14. ..

    Article Title: TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation
    Article Snippet: Transient transfection of the HEK293 cells was performed using Lipofectamine 2000 (Invitrogen) after 24 hours ( 34 ). .. Before the transfection experiment, the cells were rinsed twice with warm PBS (pH 7.4) and subsequently transfected with 2 μg of pCI-SEP-NR2B (Addgene, plasmid 23998) and mCherry-TM25 (GeneChem) on day in vitro (DIV) 14. ..

    Transfection:

    Article Title: Clustering of Tau fibrils impairs the synaptic composition of α3‐Na + /K + ‐ ATP ase and AMPA receptors
    Article Snippet: .. Plasmid Source **Amount /Transfection pCI-SEP-NRI (Addgene item #23999) (Kopec et al, 2006) 0.75μg pCI-SEP-NR2B (Addgene item #23998) (Kopec et al, 2006) 0.75μg pCI-SEP-GluR1 (Addgene item #24000) (Kopec et al, 2006) 0.5μg pCI-SEP GluR2(R) (Addgene item #24001) (Kopec et al, 2006) 0.5μg α3-NKA-Dendra2 SEP replaced with Dendra2 (Azarias et al, 2013) 0.5μg mRFP-Clc (Clathrin) (Addgene item #14435) (Tagawa et al, 2005) 0.4μg SEP = Super-ecliptic pHluorin Cell-surface biotinylation Cell surface biotinylation experiment was performed using Pierce Cell Surface Protein Isolation Kit (Thermo Fischer Scientific) as per manufacturer’s instructions. ..

    Article Title: TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation
    Article Snippet: Transient transfection of the HEK293 cells was performed using Lipofectamine 2000 (Invitrogen) after 24 hours (34). .. Before the transfection experiment, the cells were rinsed twice with warm PBS (pH 7.4) and subsequently transfected with 2 μg of pCI-SEP-NR2B (Addgene, plasmid 23998) and mCherryTM25 (GeneChem) on day in vitro (DIV) 14. ..

    Article Title: TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation
    Article Snippet: Transient transfection of the HEK293 cells was performed using Lipofectamine 2000 (Invitrogen) after 24 hours ( 34 ). .. Before the transfection experiment, the cells were rinsed twice with warm PBS (pH 7.4) and subsequently transfected with 2 μg of pCI-SEP-NR2B (Addgene, plasmid 23998) and mCherry-TM25 (GeneChem) on day in vitro (DIV) 14. ..

    Isolation:

    Article Title: Clustering of Tau fibrils impairs the synaptic composition of α3‐Na + /K + ‐ ATP ase and AMPA receptors
    Article Snippet: .. Plasmid Source **Amount /Transfection pCI-SEP-NRI (Addgene item #23999) (Kopec et al, 2006) 0.75μg pCI-SEP-NR2B (Addgene item #23998) (Kopec et al, 2006) 0.75μg pCI-SEP-GluR1 (Addgene item #24000) (Kopec et al, 2006) 0.5μg pCI-SEP GluR2(R) (Addgene item #24001) (Kopec et al, 2006) 0.5μg α3-NKA-Dendra2 SEP replaced with Dendra2 (Azarias et al, 2013) 0.5μg mRFP-Clc (Clathrin) (Addgene item #14435) (Tagawa et al, 2005) 0.4μg SEP = Super-ecliptic pHluorin Cell-surface biotinylation Cell surface biotinylation experiment was performed using Pierce Cell Surface Protein Isolation Kit (Thermo Fischer Scientific) as per manufacturer’s instructions. ..

    In Vitro:

    Article Title: TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation
    Article Snippet: Transient transfection of the HEK293 cells was performed using Lipofectamine 2000 (Invitrogen) after 24 hours (34). .. Before the transfection experiment, the cells were rinsed twice with warm PBS (pH 7.4) and subsequently transfected with 2 μg of pCI-SEP-NR2B (Addgene, plasmid 23998) and mCherryTM25 (GeneChem) on day in vitro (DIV) 14. ..

    Article Title: TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation
    Article Snippet: Transient transfection of the HEK293 cells was performed using Lipofectamine 2000 (Invitrogen) after 24 hours ( 34 ). .. Before the transfection experiment, the cells were rinsed twice with warm PBS (pH 7.4) and subsequently transfected with 2 μg of pCI-SEP-NR2B (Addgene, plasmid 23998) and mCherry-TM25 (GeneChem) on day in vitro (DIV) 14. ..



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